Product Class: Other

Phusion™ HF Buffer Pack

Product Introduction

Create optimal reaction conditions for high fidelity amplification

  • The default buffer for use with Phusion High Fidelity DNA Polymerase
  • Supplied at 5X concentration
Catalog # Size Concentration
B0518S 6 ml 5 X

Product Information

Description

New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific™ Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.
This product is related to the following categories:
Phusion™ High-Fidelity DNA Polymerases,
Reaction Buffers,
Buffers,
This product can be used in the following applications:
Long Range PCR,
Hot Start PCR,
Fast PCR,
High-Fidelity PCR,
Specialty PCR,
PCR,
Routine PCR

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • B0518S     -20    
      Phusion™ HF Buffer Pack B0518SVIAL -20 4 x 1.5 ml 5 X
      MgCl2 solution B0510AVIAL -20 2 x 1.5 ml 50 mM
      DMSO B0515AVIAL -20 1 x 0.5 ml 100 %

Properties & Usage

Protocols, Manuals & Usage

Usage & Guidelines

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. What are the differences between the various available Phusion® products?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Licenses:

Phusion™ DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. 
PHUSION™ and THERMO SCIENTIFIC™ are trademarks and property of Thermo Fisher Scientific.


Notice to purchaser: 
The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.

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