DNA/RNA preservation, sample storage and lysis solution.
Helps to protect and stabilize RNA and DNA in samples to minimize the need for immediate sample processing.
Compatible with various nucleic acid extraction kits without the need for sample precipitation or reagent removal.
Product Information
The Monarch StabiLyse DNA/RNA Buffer is a dual-function reagent for nucleic acid stabilization and sample lysis. This proprietary formulation preserves the integrity of DNA and RNA in samples during storage and it also enables effective sample lysis from a wide variety of eukaryotic and prokaryotic sample types. Samples can be stored in the Monarch StabiLyse DNA/RNA Buffer at the point of harvest for short and long term without the need for immediate processing. The Monarch StabiLyse DNA/RNA Buffer is unique in that it does not require removal of this reagent or sample precipitation.
The Monarch StabiLyse DNA/RNA Buffer is ideal for performing sample homogenization and mechanical disruption. The extracted nucleic acids are suitable for a variety of downstream applications including PCR, sequencing, and more.
PROPERTIES
Recommended use
Storage reagent for nucleic acid preservation and for sample lysis
Compatible with
DNA and RNA extractions
Sample precipitation/reagent removal before extraction
Not required –preserved samples ready for further processing and downstream applications
Sample storage recommendations
At 25°C: up to 1 week
At 4°C: up to 4 weeks
At -20°C or -80°C: 4 weeks or more
Compatible with downstream applications
PCR, sequencing, and more
Figure 1: Monarch StabiLyse DNA/RNA Buffer preserves DNA integrity during tissue sample storage10 mg pieces of rat liver were stored in 100 µl Monarch StabiLyse DNA/RNA Buffer at the time of harvest and placed at room temperature, 4°C and –80°C. DNA extraction was performed using Monarch® Spin gDNA Extraction Kit (NEB #T3010) following the tissue protocol. Isolated gDNA was run on Genomic DNA ScreenTape® on Agilent® TapeStation® 4200 (Agilent Technologies). High quality DNA (> 8.0) was observed after sample storage for 1 week at room temperature, 4 weeks at 4°C and 4 weeks at –80°C. Figure 2: Monarch StabiLyse DNA/RNA Buffer preserves RNA integrity during tissue sample storage10 mg pieces of rat liver were stored in 200 µl Monarch StabiLyse DNA/RNA Buffer at the time of harvest and placed at room temperature, 4°C and –80°C. RNA extraction was performed using Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110) following the tissue protocol. Isolated RNA was run on RNA ScreenTape on Agilent TapeStation 4200 (Agilent Technologies). High quality RNA (> 8.0) was observed after sample storage for 1 week at room temperature, 4 weeks at 4°C and 4 weeks at –80°C. Figure 3: Monarch StabiLyse DNA/RNA Buffer protects RNA against degradationPellets of 1 million HeLa cells were resuspended in either Monarch StabiLyse DNA/RNA Buffer or Phosphate-buffered saline (PBS) and incubated at 37°C for 3 hours. RNA extraction was performed using Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110). Isolated RNA was run on RNA ScreenTape on Agilent TapeStation 4200 (Agilent Technologies). Cells resuspended in Monarch StabiLyse DNA/RNA Buffer showed intact RNA compared to PBS after heat exposure indicating protection against RNA degradation.
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Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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