Lambda DNA (dam-)
Product information| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
N3013S |
Lambda DNA (N6-methyladenine-free) |
250 µg ( 500 µg/ml ) | - | Unavailable in your region | |
N3013L |
Lambda DNA (N6-methyladenine-free) |
1.250 µg ( 500 µg/ml ) | - | Unavailable in your region |
Lambda DNA (dam-)
- Lambda DNA is a commonly used DNA substrate
- Isolated from a dam- E.coli strain, this DNA can be digested with restriction endonucleases that are sensitive to dam methylation
- View sequence details
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Product Information
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (dam-) is 48,502 base pairs in length. Source
The phage is isolated from a heat-inducible E. coli(dam-) lambda lysogen. The DNA is isolated from purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0)and 1 mM EDTA.- This product is related to the following categories:
- DNA Plasmids & Substrates
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Reagents Supplied
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Properties & Usage
Storage Buffer
10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C -
Related Products
Companion Products
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Product Notes
- The advantage of this DNA is that it gives complete digests with those restriction endonucleases (BclI, MboI, DpnII, etc.) whose recognition sites are partially modified by most E. coli strains.
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References
- Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
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FAQs & Troubleshooting
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Citations & Technical Literature
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Citations
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Quality, Safety & Legal
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Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here. -
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] -
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- N3013S_L_v1_0481401
- N3013S_L_v1_0481407
- N3013S_L_v1_0481412
- N3013S_L_v1_0491412
- N3013S_L_v1_0491505
- N3013S_L_v1_0491511
- N3013S_L_v1_0491603
- N3013S_L_v1_0491608
- N3013S_L_v1_0491612
- N3013S_L_v1_0491704
- N3013S_L_v1_0491705
- N3013S_L_v1_0491707
- N3013S_L_v1_0501802
- N3013S_L_v1_0501804
- N3013L_v1_10007983
- N3013L_v1_10019245
- N3013S_v1_10019247
- N3013L_v1_10032407
- N3013L_v1_10035202
- N3013S_v1_10032410
- N3013L_v1_10055063
- N3013S_v1_10058088
- N3013L_v1_10067002
- N3013S_v1_10067003
- N3013L_v2_10071277
- N3013S_v2_10089320
- N3013L_v2_10114564
- N3013L_v2_10115054
- N3013S_v2_10119138
- N3013L_v2_10139395
- N3013S_v2_10140439
- N3013L_v2_10140441
- N3013S_v2_10157758
- N3013L_v2_10173504
- N3013L_v2_10198161
- N3013L_v2_10200180
- N3013S_v2_10207172
- N3013L_v2_10221749
- N3013S_v2_10250202
- N3013S_v2_10275288
- N3013L_v2_10275287
- N3013L_v2_10288671
- N3013S_v2_10285875
- N3013S_v2_10303203
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Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. -
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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